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Conference Agenda


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DAY 1 (Sept. 15, Monday)

2 - 4 PM. Registration

4 PM. Welcome

Douglas Bartlett, Conference Chair

4:15 PM. Opening Scientific Presentations

“Effect of hydration, temperature and pressure on protein dynamics”
Akio Kitao
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan

4:45 PM.

Title pending
Bradford Powell
Division of Bacteriology, USAMRIID, Fort Detrick, Frederick, Maryland, USA

5:15 - 7:30 PM. Opening Reception

Anfinsen’s

DAY 2 (Sept. 16, Tuesday)

Session 1: Pressure effects on protein structure and folding

Session Chair: Akio Kitao

8:30 AM. “Reversing the Anfinsen’s dogma by pressure”

Kazuyuki Akasaka
High Pressure Protein Research Center, Kinki University, Wakayama, Japan

8:55 AM. “Unfolding of three extrinsic proteins of spinach photosystem II (33kDa, 23kDa and 17kDa protein) induced by pressure”

Kangcheng Ruan
Key Laboratory of Proteomics, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai, China

9:20 AM. “The secondary structure of pressure- and heat-induced protein aggregates and amyloid fibrils studied by high pressure FTIR spectroscopy”

Yoshihiro Taniguchi
Department of Applied Chemistry, Ritsumeikan University, Japan

9:45 - 10:25 AM ========Coffee break and group photo=======

Session 2: Pressure effects on lipids

Session Chair: Kangcheng Ruan

10:25 AM. “Pressure induced phase transitions in triacylglycerides”

Peter Ferstl
Department of Fluid Mechanics and Process Automation, Technical University of Munich, Center of Life and Food Sciences, Freising, Germany

10:50 AM. “Pressure-induced interdigitation of asymmetric phospholipids bilayers: effect of vesicle size on the prodan fluorescence”

Hitoshi Matsuki
Department of Life System, Institute of Technology and Science, The University of Tokushima, Tokushima, Japan

11:15 AM. “High pressure modulated transport and signaling functions of membrane proteins”

Rudi Vogel
Department of Microbiology, Technical University of Munich, Freising, Germany

11:40 PM. “Exploring the Configurational and Free Energy Landscape of Biomolecules under Extreme Conditions: From Model Biomembranes to Proteins”

Roland Winter
Dortmund University of Technology, Dortmund, Germany

12:05 - 1:05 PM ==============Lunch break===============

Session 3: HP enzymology

Session Chairs: Dmitri R. Davydov and Gaston Hui Bon Hoa

1:05 PM. “Pressure couples protein structure to function”

Sol Gruner
Physics Department and the Cornell High Synchotron Source (CHESS), Cornell University, Ithaca, NY, USA

1:30 PM. “Hydrostatic pressure affects the conformational equilibrium of tryptophan synthase from Salmonella typhimurium”

Robert Phillips
Departments of Chemistry and of Biochemistry and Molecular Biology, University of Georgia, GA, USA

1:55 PM. “Pressure perturbation studies of enzyme-substrate interactions with lifetime fret and absorbance spectroscopy: dissecting the mechanisms of cytochrome P450 allostery”

Dmitri Davydov
Skaggs School of Pharmacy and Pharmaceutical Sciences, UC San Diego, CA, USA

2:20 PM. “Conformational flexibility of active site and ligand migration dynamics as revealed by high pressure studies on heme proteins”

Gaston Hui Bon Hoa
INSERM-U779, Le Kremlin Bicêtre, France

2:45 - 3:15 PM ============Coffee break ================

3:15 PM. “Enzymes from the piezophilic bacteria are adapted to the deep-sea environments”

Chiaki Kato
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan

3:40 PM. “Functional and biological characterization of the Escherichia coli MRR protein”

Christiaan Michiels
Laboratory of Food Microbiology, Catholic University of Leuven, Belgium

4:05 PM. Poster session 1

DAY 3 (Sept. 17, Wednesday)

Session 4: High-pressure effects on metabolism and signaling

Session Chair: Fumiyoshi Abe

8:30 AM. “Evidence for high pressure growth and metabolism decoupling in microorganisms: Implications for microbial activity in deep sediments”

Phil M. Oger
Laboratoire des Sciences de la Terre, UMR CNRS-ENS-UCBL 5570, Ecole Normale Supérieure de Lyon, Lyon, France

8:55 AM. “Unraveling the Mechanisms of Piezophily in a Deep-Sea Thermophile”

Douglas S. Clark
Department of Chemical Engineering, University of California, Berkeley, CA, USA

9:20 AM. “Membrane fatty acid and trehalose in yeast response to high hydrostatic pressure”

Patricia M.B. Fernandes
Nœcleo de Biotecnologia, Universidade Federal do Esp’rito Santo, Brazil

9:45 AM. “Genomics and metabolomics studies for the response after sub-lethal high pressure treatment to yeast Saccharomyces cerevisae”

Hitoshi Iwahashi
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Osaka, Japan

10:10 - 10:40 AM. ============Coffee break ================

10:40 AM. “Functional analysis of piezo- and psychro-adaptation in a model yeast Saccharomyces cerevisiae using 4,800 gene-deletion mutants”

Fumiyoshi Abe
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan

Session 5: Pressure pasteurization

Session Chair: Rudi F. Vogel

11:05 AM. “High pressure inactivation of food-borne viruses”

David Kingsley
USDA ARS, Delaware State University, DE, USA

11:30 AM. “Pressure inactivation of bacterial endospores in foods”

Dallas Hoover
Department of Animal & Food Sciences, University of Delaware, USA

11:55 AM - 1:00 PM ======== Lunch Break ==============

1:00 PM. “Proteins involved in Campylobacter jejuni recovery after high pressure treatment”

Clémence Bièche
Ecole Nationale Vétérinaire de Nantes, Nantes, France

1:25 PM. “Proposal of standard microorganisms for standardization of high pressure food technology”

Kaoru Obuchi
National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

1:50 PM. “Effect of petit-high pressure sterilization for fruit juice: Investigation for long time treatment with petit-high pressure carbon dioxide gas”

Tetsuji Higashi
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

Session 6: High-Pressure crystallography and imaging

Session Chair: Paul Urayama

2:15 PM. “Effects of pressure on the step velocity, two-dimensional nucleation rate and solubility of glucose isomerase crystals”

Yoshihisa Suzuki
Department of Life System, The University of Tokushima,Tokushima, Japan.

2:40 - 3:10 PM ============Coffee break ================

3:10 PM. “High pressure fixation for electron microscopy”

Gina Sosinsky,
National Center for Microscopy and Imaging Research, Dept. of Neurosciences, UC San Diego, CA, USA

3:35 PM. “High Pressure SAXS/WAXS and microRaman using a diamond anvil cell”

Ronald Gebhardt
European Synchrotron Radiation Facility, Grenoble, France

4:00 PM. Poster session 2

5:30 PM. Pressure Biosciences, Inc. Reception

DAY 4 (Sept. 18, Thursday)

Session 7: HP techniques, instrumentation and engineering

Session Chair: Edmund Ting

8:30 AM. “Equipment for High Pressure Bioscience - A scientist/engineerÕs perspective”

Edmund Ting
Pressure Biosciences, Inc., S. Easton, MA, USA

8:55 AM. “Experimental in-situ investigations of turbulence under high pressure”

Kwonyul Song
Institute of Fluid Mechanics, Friedrich-Alexander University of Erlangen-Nuremburg, Erlangen, Germany

9:20 AM. “Room temperature ionic liquids (RTILs) in high pressure bioscience and biotechnology”

Antonio Delgado
Institute of Fluid Mechanics, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany

9:45 AM. “Bridging high pressure and modern life sciences: the PCT experience in genomic and proteomic sample preparation”

F. Tao
Pressure Biosciences, Inc., S. Easton, MA, USA

10:10 - 10:40 ============Coffee break ================

10:40 AM. “Application of pressure for ultra fast protein digestion in proteomics”

Daniel López-Ferrer
Pacific Northwest National Laboratory, Richland, USA

11:05 AM. “Comparison of Pressure Cycling and Bead Beating Technologies for DNA Extraction from Polycarbonate Membranes for Water Quality Analysis”

Yiping Cao
Southern California Coastal Water Research Project, Costa Mesa, CA, USA

11:30 AM. “High-pressure, fluorescence-based sensing of calcium ions”

Paul Urayama
Department of Physics, Miami University, OH, USA

11:55 am - 1:00 PM ====== Lunch break ======

Session 8. Deep-sea life at HP

Session Chairs: Gail P. Ferguson and Phil M. Oger

1:00 PM. “Pressure effects on mineralization of the organic matter by prokaryotes in meso and bathypelagic waters of the Ocean”

Christian Tamburini
Université de la Méditerranée, Centre d'Océanologie de Marseille, Marseille, France

1:25 PM. “Effect of hydrostatic pressure on the formation of intracellular lipid bodies in the marine piezotolerant oil-degrading bacterium Marinobacter aquaeolei #5”

Vincent Grossi
Paléoenvironnements & Paléobiosphère, Université Claude Bernard Lyon 1, Villeurbanne, France

1:50 PM. “Effects of Prestige fuel oil on macro- and micro-benthic communities of deep-sea Mediterranean sediments near the ANTARES underwater neutrino telescope”

Philippe Cuny
Université de la Méditerranée, Centre d'Océanologie de Marseille, Marseille, France

2:15 PM. “Fungi in deep-sea sediments”

Samir Damare
National Institute of Oceanography, Dona Paula, Goa, India

2:40 PM - 3:10 ============Coffee break ================

3:10 PM. “Phylogenetic diversity of cultured and yet-uncultured fungi from deep-sea sediments”

Purnima Singh
National Institute of Oceanography, Dona Paula, Goa, India

3:35 PM. “Diversity of Actinobacteria cultured from deep-sea sediments collected in China’s first ever round-the-world cruise”

Jun Xu
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, Fujian, China

4:00 PM. “Isolation and characterization of a unique obligately piezophilic hyperthermophilic Archaeon from a deep-sea hydrothermal vent”

Mohamed Jebbar
Laboratory of Microbiology of Extreme Environments, University of Western Bretagne, Brest, France

4:25 PM. “Investigating the role of polysaccharides in the high pressure- and cold-adapted growth of Photobacterium profundum SS9”

Gail P. Ferguson-Bradley
School of Medicine and Dentistry, Institute of Medical Sciences, University of Aberdeen, United Kingdom

4:50 PM. “Piezo-tolerance of the cytoskeletal structure in cultured deep-sea eel cells using DNA transfection and protein introduction techniques”

Sumihiro Koyama
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan

6:30 PM. Conference Dinner

DAY 5 (Sept. 19, Friday)

Session 9: High Pressure Effects on Food

Session Chairs: Antonio Delgado and Kazutaka Yamamoto

8:30 AM. “Thermal conductivity and density of plant oils and aqueous sugar solutions under high pressure”

Cornelia Rauh
Institute of Fluid Mechanics, University of Erlangen-Nuremburg, Erlangen, Germany

8:55 AM. “Molecular effects of high pressure processing on food studied by Resonance Raman”

Volker Heinz
German Institute of Food Technologies, Quakenbrück, Germany

9:20 AM. “Microscopic in situ analysis of starch behavior under high pressure conditions”

Marta Orlowska
GEPEA / ENITIAA, Nantes, France

9:45 AM. “Pressure-Assisted Thermal Processing: Key Engineering Properties”

V.M. Balasubramaniam
Department of Food Science and Technology, Department of Food Agricultural and Biological Engineering, The Ohio State University, Columbus, OH, USA

10:10 - 10:40 AM ============Coffee break ================

10:40 AM. “Starch content and temperature as factors affecting pressure gelatinization of potato starch”

Kazutaka Yamamoto
National Food Research Institute, Tsukuba, Japan

11:05 AM. “Modelling and numerical simulation of thermofluid dynamics and biochemical reactions in high pressure processes of liquid media”

Cornelia Rauh
Institute of Fluid Mechanics, University of Erlangen-Nuremburg, Erlangen, Germany

11:30 AM. “Effect of combined high pressure-temperature treatments on the texture of sausage meat for boiled sausages”

Volker Heinz
German Institute of Food Technologies, Quakenbrück, Germany

11:55 AM. Closing remarks

Douglas Bartlett
Scripps Inst. of Oceanography

12:10 am - 1:10 PM ====== Lunch ======

Poster Session I:

  1. “High Pressure Cryocooling for Macromolecular Crystallography”

    Chae Un Kim
    Physics Department and the Cornell High Synchotron Source (CHESS), Cornell University, Ithaca, NY, USA

  2. “X-ray structure analysis of glucose isomerase crystals prepared under high pressure”

    Masayuki Tsukamoto
    Department of Chemical Science and Technology, The University of Tokushima, Japan

  3. “Solubility measurements by in situ observation of the steps of tetragonal lysozyme crystals under high pressure”

    Takahisa Fujiwara
    Department of Chemical Science and Technology, The University of Tokushima, Tokushima, Japan.

  4. “Direct observation of microtubule depolymerization in vitro at high-pressure conditions”

    Masayoshi Nishiyama
    Department of Chemistry, Graduate School of Science, Kyoto University, Japan

  5. “Crystal structure of dihydrofolate reductase from the deep-sea bacterium Moritella profunda”

    Kazuyuki Akasaka
    Department of Biotechnological Science, Kinki University, Japan

  6. “Compaction of plasmid DNA by high hydrostatic pressurization and application for gene transfection”

    Tsuyoshi Kimura
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan

  7. “Effect of pressure on the bilayer phase transition of asymmetric lipids with an unsaturated ACYL chain”

    Shoji Kaneshina
    Department of Life System, Institute of Technology and Science, The University of Tokushima, Tokushima, Japan

  8. “Effect of cholesterol on the pressure-induced interdigitation of DPPCbilayer membrane”

    Nobutake Tamai
    Department of Life System, The University of Tokushima,Tokushima, Japan

  9. “Basic aspects of phase change phenomena under high pressure - theoretical and experimental approach”

    Leszek Kulisiewicz
    Institute of Fluid Mechanics, University of Erlangen-Nuremburg, Erlangen, Germany

  10. “Recovery of tobacco by-2 cells after treatment of high hydrostatic pressure”

    Masataka Kusube
    Department of Material Science, Wakayama National College of Technology, Wakayama, Japan

  11. “Reduction of immunoreactivity of ovomucoid upon combined high pressure and proteolytic treatment”

    Sumiko Odani
    Department of Living Sciences and Technology, Niigata University, Niigata, Japan

  12. “Pressure inactivation of Escherichia coli in cashew apple juice”

    Vera Lúcia Valente Mesquita and Maria Lúcia Lopes
    Josué de Castro Institute of Nutrition, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

  13. “The effect of rpoS gene inactivation on resistance of Escherichia coli to high hydrostatic pressure”

    Rudi F. Vogel.
    Technische UniversitŠt MŸnchen, Freising, Germany.

Poster Session II:

  1. “Pressure response of brewing yeast strains”

    Patricia M. B. Fernandes
    Nœcleo de Biotecnologia, Universidade Federal do Esp’rito Santo, Brazil.

  2. “Development of food sterilization equipment by oxygen-nitrogen gas hybrid pressurization system”

    Katsuhiro Tamura
    Department of Life System, Institute of Technology and Science, The University of Tokushima, Japan

  3. “Effects of some compressed unsaturated hydrocarbon gases on yeast growth”

    Satoshi Kawachi
    Department of Life System, Institute of Technology and Science, The University of Tokushima, Japan

  4. “Novel imaging techniques for bacteria at pressure - controlling more than just pressure”

    S. Lucas Black
    School of Biology & School of Physics, The University of Edinburgh, United Kingdom

  5. “High pressure adaptation of muscle proteins from deep-sea fishes, Coryphaenoides yaquinae and C. armatus”

    Takami Morita
    National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japan

  6. “The role of DiaA and SeqA homologues in the high pressure growth of Photobacterium profundum SS9”

    Ziad El-Hajj
    Institute of Structural and Molecular Biology & Center for Science at Extreme Conditions, The University of Edinburgh, United Kingdom

  7. “Comparative genomics of piezophilic ribosomes”

    Ian Kerman
    Scripps Inst. Oceanography, La Jolla, CA, USA

  8. “Functional and Structural Characterization of Piezophilic Flagellar Motility”

    Emiley Eloe
    Scripps Inst. Oceanography, La Jolla, CA, USA

  9. “Thermogravimetric study of water state in wheat starch gels obtained under high pressure”

    Marta Orlowska
    GEPEA / ENITIAA, Nantes, France

  10. “Transitiometric analysis of water content influence on thermal and volumetric effects of wheat starch gelatinization under 10 MPa”

    Marta Orlowska
    GEPEA / ENITIAA, Nantes, France

  11. “The effects of high hydrostatic pressure on ginsenoside contents in Korean red ginseng”

    Shin Chang-sik
    Dongwon F&B research center, Jungwon-gu, Sangdaewon-dong, Seongnam-si, Gyunggi-do, South Korea

  12. “The consumer preference for pressurized mango juice”

    Rosires Deliza
    Federal Rural University of Rio de Janeiro, DTA, Seropédica - RJ, Brazil.

  13. “Influence of the high pressure of fresh catfish on its sensory and microbiological properties during the storage at 4 ¡C”

    Volker Heinz
    German Institute of Food Technologies, Quakenbrück, Germany

  14. “Combined pressure temperature effects on lycopene stability and isomerization in tomato juice”

    Rockendra Gupta
    Department of Food Science and Technology, The Ohio State University, Columbus, OH, USA

  15. “Variation of §-D-glucans extracted from medicinal mushrooms treated by High Hydrostatic Pressure”

    Kim Young-Kyung
    Dongwon F&B Research Center, Gyunggi-do, South Korea


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